Extracellular matrix requirements for gastrointestinal organoid cultures

Organoids are a new class of biological model systems that have garnered significant interest in the life sciences. When provided with proper 3D matrix and biochemical factors, stem cells can self-organize form tissue-specific organoids. Thus far, there has been substantial effort to identify soluble niche components essential for organoid culture; however, role solid extracellular (ECM) as an element is still largely lacking. In this review, we discuss importance ECM intestinal, hepatic, pancreatic culture how biomaterial-based approaches be used probe different properties required more physiologically translationally relevant models.